Effects of Chronic Ethanol Administration on Rat Liver Proteasome Activities: Relationship With Oxidative Stress

Fig. 2.   SDS-polyacrylamide gel electrophoresis of proteasome and peptidase activities. (A) Western blotting was performed using rabbit anti-rat proteasome antiserum as described in Materials and Methods. Lanes 1-2: purified rat liver proteasome (0.5 and 0.25 µg, respectively). Lane 3: 75 µg of proteins from supernatant obtained after the centrifugation of liver homogenate from control rats at 100,000g for 6 hours. Lane 4: 75 µg of proteins from supernatant obtained after the centrifugation of liver homogenate from control rats at 100,000g for 1 hour. A molecular-weight marker (33 kd) is indicated on the right side. (B) Peptidase activities were determined using fluorogenic peptides as described in Materials and Methods, in the same supernatants used in Western blotting, lanes 3-4.

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